An F-actin–dependent mechanism delays chromosome capture. (A) Schematics of the experimental protocol for acute depolymerization of F-actin. (B) Selected maximum-intensity z-projections from a 3D confocal time series though the oocyte’s nuclear region during chromosome congression. Chromosomes (H2B-3mEGFP) are in cyan and F-actin (3mCherry-UtrCH) in gray. Bars, 10 µm. See also Video 2. (C) Left: Pseudo-colored time projection of a 3D confocal time series of chromosome congression (labeled with H2B-3mEGFP) in control or Latrunculin B–treated oocytes. See also Video 3. Right: Plot of chromosome distance to the AP over time, for the control and Latrunculin B–treated oocytes shown on the left. (D) Chromosome capture events identified for 13 pairs of control and Latrunculin B–treated oocytes (plotted in a different color for each oocyte). (E) Histograms of the data shown in D. Time is given relative to NEBD for all panels.