Figure 5.
Figure 5. Microtubule destabilization in the DCC leads to loss of centrin-2. (a) Immunofluorescence images of retinal cryosections (P15) displaying localization of centrin-2 (green) and acetylated α-tubulin (red) marking the CC in WT and Spata7 mutant background. Representative single-cilium image displays localization of the protein signal at the base of CC in the Spata7 mutant. Bars: (main images) 5 µm; (single-cilium images) 1 µm. (b) Box plots display the IQR of localization signal length quantified for 20 cilia/protein in WT and Spata7 mutant retina (*, **, P < 0.0001; unpaired t test). The whiskers extend to data points that were >1.5× IQR away from first/third quartiles. (c) Representative 2D STORM reconstruction of the CC displaying centrin-2 (green) and acetylated α-tubulin (red) in the WT (left) and Spata7 mutant retinae (right) with localization length evident from the fluorophore intensity plots on the right. Bars, 200 nm.

Microtubule destabilization in the DCC leads to loss of centrin-2. (a) Immunofluorescence images of retinal cryosections (P15) displaying localization of centrin-2 (green) and acetylated α-tubulin (red) marking the CC in WT and Spata7 mutant background. Representative single-cilium image displays localization of the protein signal at the base of CC in the Spata7 mutant. Bars: (main images) 5 µm; (single-cilium images) 1 µm. (b) Box plots display the IQR of localization signal length quantified for 20 cilia/protein in WT and Spata7 mutant retina (*, **, P < 0.0001; unpaired t test). The whiskers extend to data points that were >1.5× IQR away from first/third quartiles. (c) Representative 2D STORM reconstruction of the CC displaying centrin-2 (green) and acetylated α-tubulin (red) in the WT (left) and Spata7 mutant retinae (right) with localization length evident from the fluorophore intensity plots on the right. Bars, 200 nm.

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