Cryo-ET of isolated rods displaying microtubule destabilization in the DCC. (a and b) Projections of tomogram slices showing CC from WT and Spata7 mutant mice (n = 4 mice/genotype assessed at P14). Bars, 150 nm. (c and d) Tomogram projections of CC cross sections from WT (c) and Spata7 mutant (d) mice. The cross-section views are along the z axis in the proximal-to-distal direction, with positions indicated by the horizontal lines in a and b displaying the PCC at the bottom and the most distal region at the top. Bars, 100 nm. (e and f) Segmented version of WT and Spata7 mutant tomograms with continuous isodense surfaces colored as green (A-microtubule), purple (B-microtubule), and red (C-microtubule). Cross-sectional segments at the DCC and PCC regions are displayed on the right showing the intact complex at the PCC and a progressive unraveling of the microtubule core toward the DCC.