Figure 1. SPATA7 interacts with members... | Rockefeller University Press

Figure 1.

$Figure 1. SPATA7 interacts with members of the NPHP–RPGR complex. (a) Distribution of mean GFP/WT Skyline peptide fold change of top candidate interacting partners of SPATA7 observed over three repeats. The asterisk signifies candidates that are enriched >1,000-fold in the GFP IP fraction in comparison with the WT control fraction. (b) The interaction between SPATA7 and representative RPGR was confirmed in vitro by the BiFC assay. HEK293T cells were transfected with either SPATA7-CVN alone (top) or together with RPGR-VC constructs (bottom). Strong fluorescence signals (green) were observed in cells expressing both SPATA7 and RPGR, which are quantified in c. Bar, 20 µm. (c) The percentage of BiFC-positive cells for all candidate-interacting partners that are known TZ module members was quantified by FACS (t test) in comparison with untransfected cells and SPATA7 alone. Error bars show means ± SEM.$

SPATA7 interacts with members of the NPHP–RPGR complex. (a) Distribution of mean GFP/WT Skyline peptide fold change of top candidate interacting partners of SPATA7 observed over three repeats. The asterisk signifies candidates that are enriched >1,000-fold in the GFP IP fraction in comparison with the WT control fraction. (b) The interaction between SPATA7 and representative RPGR was confirmed in vitro by the BiFC assay. HEK293T cells were transfected with either SPATA7-CVN alone (top) or together with RPGR-VC constructs (bottom). Strong fluorescence signals (green) were observed in cells expressing both SPATA7 and RPGR, which are quantified in c. Bar, 20 µm. (c) The percentage of BiFC-positive cells for all candidate-interacting partners that are known TZ module members was quantified by FACS (t test) in comparison with untransfected cells and SPATA7 alone. Error bars show means ± SEM.