ABCC transporters mediate cAMP release during streaming. (A) Tracks of aca⁻ cells migrating in an EZ-Taxiscan microfluidic device to EVs isolated from ACA-YFP/aca⁻ cells (bottom chamber) treated with DMSO (control), indomethacin, MK571, Ko143, cyclosporine A, or tariquidar. See Table S3 and Fig. 6 D for details. Results presented as mean ± SD of three independent experiments. ****,**, *, and ns indicate P < 0.0001, P < 0.002, P < 0.03, and P > 0.12, respectively. Statistical analysis was performed as described in the legend of Fig. 6. (B) Bright-field images of micropipette streaming assay showing the response of WT cells treated with DMSO, indomethacin, MK571, Ko143, cyclosporine A, and tariquidar to a micropipette filled with 1 µM cAMP. Drugs were added 1 h before the start of the assay and were present during entire assay. Images were taken 20 min after adding the micropipette to the cells. Images representative of three independent experiments.