Figure 4.
Figure 4. MS analysis of D. discoideum EVs. (A) Venn diagram (upper image) depicting the number of unique and shared proteins between EVs derived from either ACA-YFP/aca− or WT cells. Split bar graph (lower image) showing the percentage of peptide hits on shared proteins and unshared proteins in EVs derived from either ACA-YFP/aca− or WT cells. (B) Percentage of exosomal and nonexosomal proteins in EVs derived from either ACA-YFP/aca− or WT cells. Results presented as mean ± SEM of three independent experiments. (C) PSM/protein of canonical and noncanonical proteins in EVs derived from either ACA-YFP/aca− or WT cells. Results presented as mean ± SEM of three independent experiments. (D and E) Pie charts depicting the mean percentages of proteins from EVs derived from either WT or ACA-YFP/aca− cells annotated in dictyBase as vesicular, nuclear, mitochondrial, or from the ER/Golgi network or as cytoplasmic, membranous, or extracellular (n = 3).

MS analysis of D. discoideum EVs. (A) Venn diagram (upper image) depicting the number of unique and shared proteins between EVs derived from either ACA-YFP/aca− or WT cells. Split bar graph (lower image) showing the percentage of peptide hits on shared proteins and unshared proteins in EVs derived from either ACA-YFP/aca− or WT cells. (B) Percentage of exosomal and nonexosomal proteins in EVs derived from either ACA-YFP/aca− or WT cells. Results presented as mean ± SEM of three independent experiments. (C) PSM/protein of canonical and noncanonical proteins in EVs derived from either ACA-YFP/aca− or WT cells. Results presented as mean ± SEM of three independent experiments. (D and E) Pie charts depicting the mean percentages of proteins from EVs derived from either WT or ACA-YFP/aca− cells annotated in dictyBase as vesicular, nuclear, mitochondrial, or from the ER/Golgi network or as cytoplasmic, membranous, or extracellular (n = 3).

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