Rga3 is a paralog of Rga4 and contributes to cellular dimensions. (A) Medial-plane inverted images of indicated strains stained with calcofluor. Bar, 3 µm. (B) Average cell length and width of indicated strains grown in EMM-ALU at 25°C (n = 3 experiments; n > 145 cells each). Error bars show SD. Student’s t test P values are indicated. (C) Phylogenetic tree of ClustalW alignment of selected RhoGAP domain–containing proteins from Ascomycetes (purple) and Basidiomycetes (yellow) species. The Taphrinomycotina group to which fission yeasts belong is highlighted. Domain architecture (derived from SMART analysis) is shown on the right. Note that the C1 domain is present in Basidiomycetes and some Taphrinomycotina sequences but is absent in all other ascomycete sequences.