CHE12 localizes near the ends of B-tubules. (A and B) SR-SIM immunofluorescence imaging of CHE12A-GFP in growing cells before and 30 min after low pH–induced deciliation (during cilia regeneration; green, polyG tubulin; red, anti-GFP; bars: 20 µm [main], 5 µm [inset]). In A, the arrow shows a short (growing) cilium with CHE12A-GFP at the tip. Such cilia are numerous in the cell after deciliation during cilia regeneration (B). (C) An SR-SIM immunofluorescence image of CHE12B-mNeon-6xMyc (green, polyG tubulin; red, 6xMyc; bars: 20 µm [main], 10 µm [inset]). White arrow points to CHE12B at the ciliary tip. The graph shows average fluorescence intensity over the cilium length (error bars are standard deviations, n = 4 cilia; A.U., arbitrary units). White arrowheads mark the punctate signals along the cilium shaft. (D) A single TIRFM image frame taken from Video 2. The positions of three ciliary shafts are outlined by red lines, and the tips of these cilia are marked with red Ts (bar, 5 µm). (E) A kymograph corresponding to a single cilium (inside the box in D) reveals that CHE12B undergoes diffusion (bars, 1 µm and 1 s).