Kune is required for wound closure in cells not directly at the wound edge. (A–D) Confocal images of the epidermis during wound closure in control (A and B) and kune RNAi embryos (C and D) expressing GFP::Moesin labeling F-actin and Cherry::CAAX under control of the en-Gal4 promotor labeling cell membranes upon laser wounding. Top: Merged images (green, F-actin; magenta, en-Gal4). Bottom: F-actin. Images are maximum Z projections of 50 slices (17.6-µm-thick stack). (A and C) Embryos with wounds located between two en-Gal4–expressing stripes (minus). (B and D) Embryos with wound spanning one en-Gal4–expressing stripe (plus). Bar, 20 µm. (E and F) Graphs of average wound area over time in small (initial wound area <1,100 µm2) and large wounds (initial wound area >1,100 µm2) in en-Gal4–minus and –plus embryos. kune RNAi–plus embryos show a stronger phenotype than kune RNAi–minus embryos. (G) Graph of F-actin ratio at the wound edge in en-Gal4–positive versus en-Gal4–negative regions in control and kune RNAi–plus embryos at 30 mpw. An unpaired t test was used to test for significant differences between groups. ns, P > 0.05. n = 7 embryos (control); n = 7 embryos (kune RNAi). Error bars represent SEM.
