E-cad localization in control and kune mutants. (A–D) Confocal images of the epidermis during wound closure in control (A and B) and kune strong mutants (C and D) expressing ubi–E-cad::GFP (A and C) and Cherry::Moesin labeling F-actin (B and D) before and upon laser wounding. Images are maximum Z projections of 40 slices (14-µm-thick stack). Upon wounding, E-cad intensity decreases at cell boundaries facing the wound edge in controls and kune mutants (arrowheads mark the same junctions before and after wounding in each embryo). Bar, 10 µm. (E) Graph of fold change decrease in E-cad::GFP fluorescence intensity in cell boundaries at the wound edge at 10 and 30 mpw (compared with before wounding), in control (35–39 junctions from six embryos), and kune embryos (36–42 junctions from seven embryos). (F) Graph of average E-cad::GFP fluorescence intensity in cells before wounding in control (56 junctions from six embryos) and kune embryos (59 junctions from seven embryos). A two-way ANOVA with a Sidak multiple comparisons test (E) and an unpaired t test (F) were performed to test for significant differences between groups. *, P < 0.05. Error bars represent SEM.