Myosin localization is altered in kune mutant embryos. (A and B) Confocal images of the epidermis in control (A) and kune mutant embryos (B) expressing zip::GFP labeling myosin before and after laser wounding. Images are maximum Z projections of 29 slices (10-µm-thick stack) and pseudocolored in a color gradient (from lower intensities in blue to higher intensities in yellow). Myosin accumulates at the wound edge shortly after injury. Whereas in controls, myosin is maintained during the first 30 mpw, in kune mutant embryos, myosin decreases at 30 mpw (yellow arrowheads in B). Bar, 10 µm. (C) Graph of average zip::GFP intensity levels in the cortical region of cells before wounding (bw) and at the wound edge in control and kune embryos. Myosin intensity is significantly lower in kune than in controls at 30 mpw. A two-way ANOVA and a Sidak multiple comparison test were used to test for significant differences between groups. *, P < 0.05. n = 8 embryos (control); n = 9 embryos (kune). Error bars represent SEM.