kune mutants show altered wound-closure dynamics and actomyosin cable defects. (A–C) Confocal images of the epidermis in control (A), kune mild mutant (B), and kune strong mutant embryos (C) expressing Cherry::Moesin labeling F-actin after laser wounding. Images are maximum Z projections of 49 slices (17-µm-thick stack) and pseudocolored in a color gradient (from lower intensities in blue to higher intensities in yellow). In all genotypes, F-actin accumulates at the wound edge at 10 mpw, and at 20 mpw, the wound starts contracting. At 120 mpw, the wound has closed in control embryo, whereas in the kune mild embryo, the wound has not closed, and in kune strong embryo, the wound size has increased. Bar, 20 µm. (D) Graph of average time of wound closure in control and kune mild and strong mutants. Note that from the total number of embryos analyzed, five kune strong wounds did not close and thus were not considered in this analysis. (E) Graph of average initial wound area in control and kune mild and strong mutants. (F) Graph of average wound area over time shows that kune mutants have altered wound-closure dynamics when compared with controls. In kune mild mutants, wounds closed slower than in controls. In kune strong mutants, wounds stopped contracting between 20 and 40 mpw, increased their area until ∼120–180 mpw, and then decreased their wound size again. (G) Graph of average time of wound closure in control and kune mutants divided in groups according to initial wound area (small wounds = 468–1,100 µm²; large wounds = 1,101–1,820 µm²). Note that from the total number of embryos analyzed, two kune small wounds and three kune large wounds did not close and were not considered in this analysis. (H) Graph showing the number of kune embryos showing a mild and strong phenotype according to initial wound area. (I) Graph of average F-actin intensity levels in the cortical region of cells before wounding (bw) and at the wound edge in control and kune embryos. F-actin intensity is significantly lower in kune than in controls at 20 and 30 mpw. Unpaired t test corrected for multiple comparisons using the Holm-Sidak method was performed to test for significant differences between groups in D, E, and G. *, P < 0.05; **, P <0.01; ***, P < 0.001. n = 12 embryos (control); n = 10 embryos (kune mild); n = 8 embryos (kune strong); n = 8 embryos (control small); n = 9 embryos (kune small); n = 4 embryos (control large); n = 4 embryos (kune large). A two-way ANOVA and a Sidak multiple comparison test were used to test for significant differences between groups in I. **, P < 0.01. n = 4 embryos (control); n = 5 embryos (kune). Error bars represent SEM.