Mutants for SJ-related proteins show wound-closure defects. (A and B) WT embryo (A) and kune mutant embryo (B) 16 h after wounding. Bar, 100 µm. (C) Graph shows the percentage of open wounds 16 h after wounding in WT and SJ mutant embryos. Mutants for different types of SJ components were tested: claudin transmembrane proteins (blue), other transmembrane adhesion proteins (green), intracellular scaffolding proteins (orange), Ly6 family proteins that regulate SJ complex assembly (magenta), and proteins that regulate SJ localization (red). Fisher’s exact test shows that all these SJ mutants have significantly higher percentages of open wounds when compared with controls. ***, P < 0.0001. See figure for the number of embryos for each genotype.