Mechanical stress induces EHD2 nuclear translocation. (A and B) Representative wide-field immunofluorescence (left) and quantification (right) of the nuclear translocation of endogenous EHD2 (A) but not cavin1 (B) in HeLa cells after 30 min of cyclic stretch. (C) Representative wide-field immunofluorescence (left) and quantification (right) of endogenous EHD2 and Cav1 localization in HeLa cells under resting (Iso), after 5 min of 30 mOsm hypo-osmotic shock (Hypo), and 5 min after return to iso-osmotic conditions (Rec). (D) Immunoblot analysis (left) and quantification (right) of equal amounts of nuclear, cytoplasmic and cell membrane extracts after hypo-osmotic shock for the indicated times in MLEC cells having caveolae (WT) or not (Cav1^−/−). Scale bar = 10 µm; n ≥ 3 independent experiments; *, P < 0.05; ***, P < 0.001; ****, P < 0.0001; in A and B, two-tailed t test; data are representative of three experiments, mean ± SEM; in C, Bonferroni’s multiple comparison test; data are mean ± SEM; numbers of cells are indicated on the graphs; in D, Dunn’s multiple comparison test; n = 3; data are mean ± SEM.