Lnx1 sustains stability of EphB receptors. (A) Analysis of degradation of EphB1 and EphB2 after addition of inhibitors of proteasome or lysosome in primary hippocampal neurons from WT and Lnx1^−/− mice. *, EphB1; #, EphB2. Quantitative results of three biological replicates are shown. (B) Analysis of endogenous EphB2 protein level in MEF cells infected with lentivirus containing mock, Lnx1, or Lnx1-△PDZ2 in the presence or absence of proteasome inhibitor MG132. Quantitative results of three biological replicates are shown. (C) Analysis of EphB protein levels in primary hippocampal neurons from Lnx1^−/− mice infected with lentivirus containing mock, Lnx1, Lnx1-△NT, Lnx1-△PDZ1, or Lnx1-△PDZ2. Quantitative results of four biological replicates are shown. Means ± SEM; two-way ANOVA with Tukey’s post hoc test (A and C) or one-way ANOVA with Tukey’s post hoc test (B); *, P < 0.05; **, P < 0.01; ***, P < 0.001; ^###, P < 0.001. IB, immunoblot.