Loss of Ajuba enhances the dorsal closure defects in cell adhesion mutants. (A) Ajuba-GFP localization in a living embryo during dorsal closure. (B–G) Stills from time-lapse videos of stage 14 embryos expressing β-catenin-GFP. WT (B), E-cadherin heterozygote (shg²/+) (C), Ajuba^54MZ maternal/zygotic mutant (D), Ajuba^IIMZ maternal/zygotic mutant (E), Ajuba^54MZ maternal/zygotic mutant that is also heterozygous for E-cadherin (F; Ajuba^54MZ; shg²/+), and Ajuba^IIMZ maternal/zygotic mutant that is also heterozygous for E-cadherin (G; Ajuba^IIMZ; shg²/+). The lateral epidermis (annotated in blue) is connected to the amnioserosa (annotated in yellow) in WT but becomes separated in Ajuba mutants. (H) Percentage of embryos showing no defect or mild, moderate, or severe dorsal closure phenotypes. Images are dorsal views, anterior left. Bars, 30 µm. See also Fig. S4 and Videos 5–10.