Ajuba mutants have defects in junctional protein localization at sites of multicellular adhesion. (A and B) WT and Ajuba mutant embryos immunostained for β-catenin (A) or α-catenin (B). Gaps devoid of β-catenin and α-catenin occur at rosette vertices in Ajuba mutants (arrowheads). Yellow boxes (top) show the location of cells in the high-magnification images (bottom). (C) Embryos immunostained for E-cadherin and Myo-GFP. In WT embryos, E-cadherin and myosin in neighboring cells are closely apposed at cell interfaces. In Ajuba mutants, E-cadherin signal is disrupted at vertical edges in forming rosettes, and myosin separates into two parallel bands (arrowheads). (D) Z slices of rosettes from confocal images in B of WT and Ajuba⁵⁴ mutant embryos immunostained for α-catenin. (E) Cross-sections of WT and Ajuba mutant embryos immunostained for β-catenin. Images are anterior left, ventral down in A–D, and apical up in E. Bars: (A–C) 10 µm; (D and E) 5 µm. All embryos are shown at stage 8. See also Fig. S4.