Ajuba mutants display defective cell adhesion in rosettes. (A and B) Images (A) and schematics (B) of rosette formation through simultaneous or sequential contraction. Shrinking edges indicated by red and green arrowheads; rosettes highlighted in yellow. (C) Percentage of shrinking edges that formed rosettes through simultaneous or sequential contraction. (D) Images of rosettes from videos of WT and AjubaII mutant embryos. Neighboring cells are closely apposed during rosette formation in WT, but gaps appear between cells in Ajuba mutants (white arrowhead). Colored dots indicate cells in rosettes. (E) Percentage of rosettes that displayed gaps at rosette vertices. (F) Lifetime of high-order vertices in T1 processes (T1s) or rosettes. (G) Percentage of shrinking edges that formed vertices that did not resolve. The mean ± SEM between embryos is shown (n = 12 embryos/genotype, 33–49 shrinking edges analyzed/embryo in C, F, and G; 7–21 rosettes analyzed/embryo in E); *, P < 0.05; **, P < 0.001; one-way ANOVA with Fisher’s least significant difference test. Cells are labeled with β-catenin-GFP. Images are anterior left, ventral down. Bar, 5 µm. See also Figs. S4 and S5 and Videos 3 and 4.
