Loss of Ajuba activity enhances higher-order rosette behaviors. (A) Schematics of T1 and rosette processes. (B) Rosette:T1 ratio, calculated as the ratio of the number of shrinking edges that joined rosettes or T1 processes during axis elongation in WT, Ajuba^II, and Ajuba⁵⁴ mutant embryos. The mean ± SEM between embryos is shown; *, P < 0.001; **, P < 0.0001; one-way ANOVA with Fisher’s least significant difference test. (C) Percentage of shrinking edges that formed four-cell vertices (T1 processes, light shading), five-cell rosettes (medium shading), or rosettes with six or more cells (dark shading; n = 12 embryos/genotype, 33–49 shrinking edges analyzed/embryo in B and C). (D) Stills from videos of WT and Ajuba mutant embryos with rosettes of five or more cells highlighted. Anterior left, ventral down. Bar, 20 µm. See also Figs. S4 and S5 and Videos 1 and 2.