Figure 4.
Figure 4. Ajuba variants are differentially regulated by tension. (A) Images of Ajuba-msVenus variants in stage 6 WT and ShroomA-overexpressing (Shroom OE) embryos. LIM only and LIM-NES images were acquired at a higher gain and in a more basal plane to show nuclear localization (LIM only) or exclusion (LIM-NES). Images are anterior left, ventral down. Bar, 10 µm. (B) Vertex enrichment of Ajuba variants in WT (gray) and Shroom OE (white) embryos. Vertex intensities were divided by the cytoplasmic intensity to calculate the vertex enrichment. The mean ± SEM between embryos is shown (n = 8 embryos/variant, 30 vertices analyzed/embryo); *, P < 0.05; **, P < 0.001 compared with full-length Ajuba (Ajuba-FL); one-way ANOVA with Fisher’s least significant difference test. See also Fig. S3.

Ajuba variants are differentially regulated by tension. (A) Images of Ajuba-msVenus variants in stage 6 WT and ShroomA-overexpressing (Shroom OE) embryos. LIM only and LIM-NES images were acquired at a higher gain and in a more basal plane to show nuclear localization (LIM only) or exclusion (LIM-NES). Images are anterior left, ventral down. Bar, 10 µm. (B) Vertex enrichment of Ajuba variants in WT (gray) and Shroom OE (white) embryos. Vertex intensities were divided by the cytoplasmic intensity to calculate the vertex enrichment. The mean ± SEM between embryos is shown (n = 8 embryos/variant, 30 vertices analyzed/embryo); *, P < 0.05; **, P < 0.001 compared with full-length Ajuba (Ajuba-FL); one-way ANOVA with Fisher’s least significant difference test. See also Fig. S3.

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