Ajuba junctional localization and planar polarity require the preLIM and LIM domains. (A) Schematic of Ajuba variants. All variants were tagged with C-terminal msVenus and expressed under the control of a maternal Gal4 driver. (B) Junctional enrichment of Ajuba variants at vertical edges (oriented at 75–90° relative to the AP axis) and horizontal edges (oriented at 0–15° relative to the AP axis) in stage 7 embryos. Edge intensities were divided by the cytoplasmic intensity to calculate the junctional enrichment. (C) Confocal images of living stage 7 embryos expressing Ajuba variants tagged with C-terminal msVenus. LIM only and LIM-NES images were acquired at a higher gain and in a more basal plane to show nuclear localization (LIM only) or exclusion (LIM-NES). Images are anterior left, ventral down. Bar, 10 µm. (D) Planar polarized localization of Ajuba variants (the background-subtracted vertical edge intensity divided by the background-subtracted horizontal edge intensity). The mean ± SEM between embryos is shown (n = 5 embryos/variant, 167–416 interfaces analyzed/embryo); *, P < 0.01; **, P < 0.001 compared with full-length Ajuba (Ajuba-FL); one-way ANOVA with Fisher’s least significant difference test. See also Fig. S3.