Sister chromatid cohesion is highly resistant to cohesin loss. (A) Experimental layout: embryos carrying different ratios of TEV-cleavable/TEV-resistant complexes were arrested in metaphase using UbcH10^C114S and subsequently injected with TEV protease to trigger acute removal of specific cohesin percentages. (B) Metaphase-arrested chromosome behavior (labeled with H2B-mRFP) monitored for 20 min after TEV protease injection in the developed strains. The last column presents a magnified view of a metaphase 20 min after TEV injection. Bars, 5 µm. (C) Sister chromatid separation in the various strains at different times after TEV injection. n = 23, 5, 11, 9, 19, 23, and 13 embryos, respectively.