Dendrite morphology in tsr mutants. (A) Defective ddaC dendritic patterns in MARCM clones for FRT^G13 tsr^N121 (n = 12) and FRT^G13 tsr^N96A (n = 12) marked by GAL4^5-40-driven UAS-Venus for labeling dendrites, with FRT^G13 tsr⁺ (n = 11) used as control MARCM. (B) Bar graph shows dendritic endpoints in the posterior dorsal region of ddaC neurons. The control (FRT^G13 MARCM) is a replicate of the control in Fig. 5 A′. (C) Sholl analysis shows dendrite intersections with concentric rings in 10-µm increments from the proximal region until the distal end. Number of neurons: 9 (control), 11 (tsr^N121), and 12 (tsr^N96A) from two to three independent experiments. (D) Immunointensity of Tsr-GFP in class IV da neuron at 72 h (n = 9) and 96 h AEL (n = 9) and in tsr-RNAi (n = 8) neurons at 72 h AEL from two to three experiments. Tsr-GFP intensities normalized to CD4-tdTomato within soma (outlined by dashed lines) were scored and shown in the bar graph (right). Tsr-GFP intensity was normalized to the neuronal marker. Significance was determined using Student’s t test. **, P < 0.01; ***, P < 0.001. Error bars represent SEM. Each dot represents a neuron.