Directed transport and streaming cooperate for robust Staufen posterior localization. Staufen staining and summary of Staufen localization patterns at stage 9 and stage 10B and Staufen distribution (normalized fluorescence intensity; mean ± 95% confidence interval) at stage 10B oocyte cortex were shown in following genotypes: control, Khc^mutA, Khc-RNAi (nos>Khc-TRiP RNAi-GL00330), Klc-RNAi (nos>Klc-TRiP RNAi-GL00535), btz-RNAi (nos>btz- TRiP.RNAi-GLC01869), Klc-RNAi + btz-RNAi, Khc^mutA +Klc-RNAi + btz-RNAi, and Khc-RNAi + Unc104-Ktail. To note: only the rescued Khc-RNAi +Unc104-Ktail stage 10B oocytes were analyzed in Staufen distribution on the oocyte cortex. Bars, 50 µm. Arrows and circular arrows stand for directed transport and streaming circulation, respectively. The widths of the arrow lines represent the level of transport or streaming.