Effects of Khc^mutA on Staufen movement. (A–D) Replacement of endogenous Khc with Khc^mutA does not affect overall Staufen movement in stage 9 oocytes. (A and B) Temporal color-coded images showing Staufen-SunTag particle movement in cytoplasmic areas of a stage 9 control oocyte (A) and a stage 9 Khc^mutA oocyte (B). (C and D) Quantification of Staufen-SunTag movement in cytoplasm of stage 9 oocytes. (C) Average velocities (mean ± 95% confidence interval) of Staufen-SunTag in cytoplasm of control oocytes and of Khc^mutA oocytes. (D) Histograms of frequency distribution (percentages) of Staufen-SunTag velocity in cytoplasm of control and of Khc^mutA oocytes. The inset shows the average velocity of fast-moving Staufen-SunTag particles (>0.1 µm/s) in cytoplasm of control and of Khc^mutA oocytes. (E–H) Replacement of endogenous Khc with Khc^mutA leads to slower movement of Staufen particles in cytoplasm of stage 10B oocytes. (E and F) Temporal color-coded images showing Staufen-SunTag particle movement in cytoplasmic areas of a stage 10B control oocyte (E) and a stage 10B Khc^mutA oocyte (F). (G and H) Quantification of Staufen-SunTag movement in cytoplasm of stage 10B oocytes. (G) Average velocities (mean ± 95% confidence interval) of Staufen-SunTag in cytoplasm of control oocytes (the same set of data as Fig. 1 C) and of Khc^mutA oocytes. (H) Histograms of frequency distribution (percentages) of Staufen-SunTag velocity in cytoplasm of control and of Khc^mutA oocytes. Bars, 50 µm.