Nck recruits dynamin to CEV. (A) Dyn II and actin are recruited to CEV in HeLa cells infected with WR. The graph shows the percentage of CEV recruiting dyn II in WR- and YdF-infected cells. (B) The graph shows the percentage of CEV with dynamin, Nck, or both proteins in HeLa cells infected with WR. (C) Quantification of the percentage of CEV recruiting dyn II in the presence (Allstar) or absence (SEPT7) of septin in WR-infected HeLa cells. (D) The graph shows the percentage of CEV recruiting dynamin in Nck parental (+/+) and knockout cells (−/−) infected with WR or the YdF virus. (E) The graph shows the percentage of CEV recruiting dyn II in WR-infected Nck knockout cells (−/−) expressing GFP-tagged Nck (WT) or mutants with defective first and second SH3 (Δ1+2) or third SH3 (Δ3) domains. Error bars represent SEM from three independent experiments in which a total 900 virus particles were analyzed in 30 cells. Bars, 5 µm. **, P < 0.01; ****, P < 0.0001.