Figure 9. The Zas1 TAD motif binds to... | Rockefeller University Press

Figure 9.

$Figure 9. The Zas1 TAD motif binds to the CHD. (A) Coomassie-stained SDS PAGE of Zas1 proteolysis products of trypsin or subtilisin digestion for the indicated time periods. The identity of the main cleavage products was determined by mapping N (open boxes/arrows) and C (closed boxes/arrows) termini using mass spectrometry. Gray lines indicate the positions of theoretical trypsin (top) or subtilisin (bottom) cleavage sites in the protein. (B) Analytical SEC before (blue curve) or after (red curve) limited proteolysis of Zas1 with trypsin. Peak fractions (gray bars) were analyzed by SDS-PAGE and Coomassie staining. The identities of the bands were determined by mapping N and C termini using mass spectrometry and are indicated in the scheme. (C) Models of the interaction of the TAD motif (red) with a helical domain (yellow) of the same Zas1 molecule (in cis) or with a second Zas1 molecule (in trans). (D) SEC-MALS analysis of purified Zas1 (mean molecular weight estimate ± min/max from two independent chromatography runs).$

The Zas1 TAD motif binds to the CHD. (A) Coomassie-stained SDS PAGE of Zas1 proteolysis products of trypsin or subtilisin digestion for the indicated time periods. The identity of the main cleavage products was determined by mapping N (open boxes/arrows) and C (closed boxes/arrows) termini using mass spectrometry. Gray lines indicate the positions of theoretical trypsin (top) or subtilisin (bottom) cleavage sites in the protein. (B) Analytical SEC before (blue curve) or after (red curve) limited proteolysis of Zas1 with trypsin. Peak fractions (gray bars) were analyzed by SDS-PAGE and Coomassie staining. The identities of the bands were determined by mapping N and C termini using mass spectrometry and are indicated in the scheme. (C) Models of the interaction of the TAD motif (red) with a helical domain (yellow) of the same Zas1 molecule (in cis) or with a second Zas1 molecule (in trans). (D) SEC-MALS analysis of purified Zas1 (mean molecular weight estimate ± min/max from two independent chromatography runs).