Figure 4. Augmin complexes exhibit... | Rockefeller University Press

Figure 4.

$Figure 4. Augmin complexes exhibit varying activities in branching MT nucleation. (A) Four augmin complexes were generated based on the model of augmin assembly and confirmed by size-exclusion chromatography using a Superdex 200 increase 10/300 GL column. The peak elution volume of each complex was determined. (B) Peak fractions were analyzed by a SDS-PAGE gel (silver staining), showing the components of each complex and stoichiometric complex formation. (C) Augmin complexes (150 nM) were added back to the augmin-depleted extract (Δ H1), and their activities were examined by branching MT nucleation assays. EB1 is shown in green and MTs in red. Bar, 10 µm. Images were taken 35 min after sample preparation. IgG is the control extract without augmin depletion.$

Augmin complexes exhibit varying activities in branching MT nucleation. (A) Four augmin complexes were generated based on the model of augmin assembly and confirmed by size-exclusion chromatography using a Superdex 200 increase 10/300 GL column. The peak elution volume of each complex was determined. (B) Peak fractions were analyzed by a SDS-PAGE gel (silver staining), showing the components of each complex and stoichiometric complex formation. (C) Augmin complexes (150 nM) were added back to the augmin-depleted extract (Δ H1), and their activities were examined by branching MT nucleation assays. EB1 is shown in green and MTs in red. Bar, 10 µm. Images were taken 35 min after sample preparation. IgG is the control extract without augmin depletion.