Augmin directly recruits γ-TuRC to MTs. (A) MT cosedimentation assays were performed with γ-TuRC and γ-TuRC plus augmin at different concentrations of MTs (2, 0.4, 0.08, and 0 µM), demonstrating augmin-dependent recruitment of γ-TuRC to MTs. (B) Quantification of MT cosedimentation assay results. MT cosedimentation ratios of γ-TuRC without and with augmin were calculated for each MT concentration using averaged signals of GCP4 from Western blots using two independent experiments. An error bar denotes ± standard deviation across replicates. (C) IF of γ-tubulin (red, 568 channel) on prepolymerized Cy5-MTs in the absence of γ-TuRC (top panel), the absence of GFP-Augmin (middle panel), or the presence of both (bottom panel). Bar, 10 µm. (D) Boxplot of γ-TuRC signal relative to MT signal. Each dot represents a single MT, pink boxes denote ± 1 standard deviation, blue lines show the 95% confidence interval, and red bars show the mean. Triple asterisks (***) denote a p-value below 0.0001. The numbers of MTs (n) were obtained from three independent experiments.