Figure 4.
Figure 4. Depletion of cholesterol specifically impairs the formation of TJs. (A) WT EpH4 cells were cultured in transwell chambers and treated with PBS (control) or 75 mM MβCD for the indicated duration, and then cells underwent TER analysis (means ± SD; n = 4). (B) WT EpH4 cells were treated with PBS (control), 25 mM MβCD, 50 mM MβCD, or 75 mM MβCD for 30 min, fixed, and costained with an anti–claudin-3 pAb and an anti–E-cadherin mAb. (C) WT EpH4 cells were treated with PBS (control) or 75 mM MβCD for 30 min, fixed, and costained with an anti–claudin-3 pAb and an anti–desmoglein-2 mAb. (D) WT EpH4 cells were treated with PBS (control) or 50 mM MβCD for 30 min, fixed, and costained with an anti–E-cadherin mAb and an antioccludin pAb. Bars, 20 µm.

Depletion of cholesterol specifically impairs the formation of TJs. (A) WT EpH4 cells were cultured in transwell chambers and treated with PBS (control) or 75 mM MβCD for the indicated duration, and then cells underwent TER analysis (means ± SD; n = 4). (B) WT EpH4 cells were treated with PBS (control), 25 mM MβCD, 50 mM MβCD, or 75 mM MβCD for 30 min, fixed, and costained with an anti–claudin-3 pAb and an anti–E-cadherin mAb. (C) WT EpH4 cells were treated with PBS (control) or 75 mM MβCD for 30 min, fixed, and costained with an anti–claudin-3 pAb and an anti–desmoglein-2 mAb. (D) WT EpH4 cells were treated with PBS (control) or 50 mM MβCD for 30 min, fixed, and costained with an anti–E-cadherin mAb and an antioccludin pAb. Bars, 20 µm.

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