RSC mutants show genetic interactions with SPB genes. (a) The indicated rscΔ strains were subjected to SGA analysis. The SGA scores for rscΔ and temperature-sensitive SPB alleles are displayed as a heat map. Positive genetic interactions have SGA scores >0.080, and negative genetic interactions have SGA scores less than −0.080. (b) The HTL1 shut-off strain was transformed with a high-copy-number plasmid expressing the indicated SPB component gene from its native promoter. Serial dilutions of each strain were spotted on medium containing galactose or glucose and grown for 3–5 d. (c) The indicated HTL1 shut-off strains from panel b were grown in liquid medium containing galactose or glucose for the specified amount of time, and ploidy was monitored by flow cytometry.