Diploidized rscΔ mutants are morphologically distinct from wild-type MATa/α diploids. (a) CalMorph software was used to analyze the yeast deletion collection. Strains were ranked by whole-cell size in budded cells containing one nucleus (C101_A1B) or by bud/axis ratio in budded cells containing one nucleus (C114_A1B) and displayed graphically. Mutants that are smaller (top) or have rounder buds (bottom) than wild type are shown in green. Mutants that are larger (top) or have more elongated buds (bottom) than wild type are shown in pink. The haploid wild-type strain is shown in white, and the rscΔ mutants are shown in yellow. Representative images of wild-type and rscΔ cells are shown. The number above or below the image represents the rank number among the 4,718 deletion mutants analyzed for C101_A1B or C114_A1B, respectively. (b) PCA was applied to the morphological dataset created for the haploid yeast deletion collection. (c) Strains with temperature-sensitive alleles of STH1 were imaged and analyzed with CalMorph and plotted in the context of the analysis in panel b. (d) The DNA contents of the indicated strains after 24 h of logarithmic growth at 23°C are plotted.