Aberrant spindle positioning in PGR mutants. (A) Percentage of cells with aberrant spindle positioning during RTG and mitosis (n = 200 each). Differences between RTG and mitosis are statistically significantly (Two-tailed Fisher’s exact test; P < 0.001) unless marked ns. (B–F) Time-lapse images showing aberrant spindle positions during RTG with time in minutes after nutrient-rich medium addition. Bars, 3 µm.