During RTG, some PGR mutants bud multiple times, and all mutants have enhanced Tyr19-phosphorylated CDK. (A) Images from a time-lapse experiment showing formation of two buds before nuclear division in hsl1Δ, gin4Δ, nap1Δ, and cla4Δ cells during RTG. Bars, 3 µm. (B) Percentage of cells that budded multiple times before nuclear division during RTG (n = 200 each). (C) Western blots with anti–Tyr19-phosphorylated CDK (CDK-P) and anti-PGK antibodies. PGK served as a loading control. Numbers indicate time points in minutes after transfer to nutrient-rich medium from meiotic prophase I. A sample from cells overexpressing Swe1 for 300 min in mitosis served as a reference standard for CDK phosphorylation.