The PGRs are dispensable during mitosis but crucial during RTG. (A and B) Images of WT and elongated bud mutants during RTG (A) and during mitosis (B). Bars, 3 µm. (C and D) Mean time of bud formation and anaphase during RTG and mitosis (n = 100 each). For RTG, time in minutes measured from transfer to nutrient-rich medium to bud formation (C) or anaphase onset (D). For mitosis, time in minutes measured from previous spindle breakdown. (E) Mean bud length during both RTG and mitosis (n = 100 for each). Differences between WT and each mutant during RTG are statistically significant for time of anaphase onset and bud length (Mann-Whitney test with computation of two-tailed exact p-value; P < 0.001). (F) Percentage of cells with elongated buds during RTG and during mitosis (n = 100 each). Differences were statistically significant (Two-tailed Fisher’s exact test; P < 0.05) unless marked ns. Error bars indicate SD.