A genetic screen isolated mutants in which polarity and cell growth are uncoordinated with nuclear division. (A) A simplified screen outline. (B–G) Time in minutes after nutrient-rich medium addition (RTG) or after the previous spindle breakdown (mitosis). (B) Time-lapse images of a swe1Δ cell during RTG that fails to polarize GTP-Ccd42 or bud yet undergoes a nuclear division. (C) Time of GTP-Cdc42 accumulation and budding in swe1Δ cells during RTG and mitosis are statistically significantly different (Mann-Whitney test with computation of two-tailed exact p-value; P < 0.0001; n = 100 cells each). (D) Time-lapse images of a swe1Δ cell during mitosis with GTP-Cdc42 accumulation, budding, and anaphase. (E) Time-lapse images of a mih1Δ cell during RTG with an elongated bud and hyperpolarized GTP-Cdc42 at the bud tip. (F) Time of GTP-Cdc42 accumulation and budding during RTG and mitosis in mih1Δ cells (n = 100 cells each). Differences are statistically significant (Mann-Whitney test with computation of two-tailed exact p-value; P < 0.0001). (G) Time-lapse images of a mih1Δ cell showing GTP-Cdc42 accumulation, budding, and nuclear division during mitosis. DIC, differential interference contrast. Bars, 3 µm.