Spindle centering is corrected during anaphase. (A) Representative time-lapse images of cells going through mitosis incubated with SiR-tubulin (green) and CellMask dye (red). The 0 time point is defined as the last frame before anaphase spindle elongation takes place. Bar, 10 µm. (B) Graph representing the mean spindle length during the metaphase-to-anaphase transition for HeLa and Kif18b-KO HeLa cells (n = 64 and 44). (C) Graph showing the displacement of the center of the spindle with respect to the center of the cell along the spindle elongation axis. Shaded areas represent 95% confidence intervals.