The efficiency of centriole disengagement scales to ploidy level. (A) Immunostaining of centrin and C-Nap1 in haploid, diploid, and tetraploid cells at mitotic exit. Whole-cell images (top) and 3× enlarged images of the centrioles (bottom). Asterisks indicate nonspecific staining of the intercellular bridge. (B) Frequencies of cells with disengaged centriole pairs (with two centrin and two C-Nap1 dots) in A. Values represent means ± SE of three independent experiments (*, P < 0.05, t test). At least 144 cells were analyzed for each condition. (C) Live imaging of GFP-centrin cells taken at 2-min intervals. Cytokinesis onset was set as 0 min. Whole-cell images of a haploid cell (top) and enlarged images of the centrioles in cells with different ploidies (bottom). (D) Time course of intercentriolar distance in C. Values represent means ± SE of at least nine cells from at least two independent experiments for each data point (at least 10 cells were analyzed for each condition). Green or magenta markers at the bottom of the graph indicate statistically significant differences between haploid and diploid or diploid and tetraploid cells, respectively (P < 0.05, t test). (E) Cumulative frequency of cells in which intercentriolar distance had reached 0.8 µm in C. At least 10 cells from at least two independent experiments were analyzed for each condition.