Centriole duplication efficiency scales to ploidy level in HAP1 cells. (A and D) BrdU incorporation after nocodazole release in cells with different ploidies. Mean ± SE of three independent experiments. At least 1,258 (A) or 818 cells (D) were analyzed for each data point. (B and E) Immunostaining of centrin in synchronized cells with different ploidies. Insets show 3× enlarged images of centrioles. Broken lines show cell boundaries. (C and F) Percentages of cells with indicated numbers of centrin foci at each time point after nocodazole release. Values represent means ± SE of three independent experiments (asterisks indicate significant differences from diploid cells; *, P < 0.05; **, P < 0.01, t test). At least 170 (C) or 87 cells (F) were analyzed for each data point. Note that centrin foci number decreased from 8 h to 12 h after nocodazole release in some cases, because some population of cells divided during that time.