Haploid cells are more susceptible to centrosome loss than diploid cells. (A) Live images of haploid or diploid GFP-centrin cells pretreated with 500 nM centrinone B for 1 d. Images were taken at 25-min intervals. Mitotic entry was set as 0 min. Arrowhead or arrow indicates mitotic death or mitotic slippage, respectively. Broken lines show cell boundaries. Asterisks mark neighboring cells. (B) Classification of mitotic defects (outer circle) sorted by centriole content (inner circle) determined by live imaging of GFP-centrin cells in A. Data are from two independent experiments. Mitotic cells that moved out of the field of view or did not exit mitosis by the end of imaging were categorized as unknown. (C) Flow cytometric analysis of DNA content in Hoechst-stained cells pretreated with or without 500 nM centrinone B for 4 d. Representative data from two independent experiments are shown.