Figure 1. Haploid-specific mitotic... | Rockefeller University Press

Figure 1.

$Figure 1. Haploid-specific mitotic defects and subsequent diploidization in HAP1 cells. (A) Flow cytometric analysis of DNA content in Hoechst-stained cells during long-term culture. Representative data from three independent experiments are shown. (B) Hoechst-stained haploid and diploid cells. (C) Live images of haploid and diploid cells taken at 5-min intervals. Nuclear envelope breakdown (NEBD) was set as 0 min. Arrowhead indicates mitotic death. Broken lines mark cell boundaries. (D and E) Distribution of cell cycle length (from one NEBD to the next NEBD) or mitotic duration (from NEBD to anaphase onset) quantified from 339 haploid and 285 diploid (D) and 1,180 haploid and 1,015 diploid cells (E), respectively. Data are from at least two independent experiments. (F) Classification of mitotic defects (outer circle) sorted by mitotic duration (inner circle) determined based on analysis of 1,181 haploid and 1,015 diploid cells in at least two independent experiments. (G) Time course of the haploid G1 fraction during long-term culture of haploid-enriched cells in A. Data from three independent experiments are compared with theoretical model simulations.$

Haploid-specific mitotic defects and subsequent diploidization in HAP1 cells. (A) Flow cytometric analysis of DNA content in Hoechst-stained cells during long-term culture. Representative data from three independent experiments are shown. (B) Hoechst-stained haploid and diploid cells. (C) Live images of haploid and diploid cells taken at 5-min intervals. Nuclear envelope breakdown (NEBD) was set as 0 min. Arrowhead indicates mitotic death. Broken lines mark cell boundaries. (D and E) Distribution of cell cycle length (from one NEBD to the next NEBD) or mitotic duration (from NEBD to anaphase onset) quantified from 339 haploid and 285 diploid (D) and 1,180 haploid and 1,015 diploid cells (E), respectively. Data are from at least two independent experiments. (F) Classification of mitotic defects (outer circle) sorted by mitotic duration (inner circle) determined based on analysis of 1,181 haploid and 1,015 diploid cells in at least two independent experiments. (G) Time course of the haploid G1 fraction during long-term culture of haploid-enriched cells in A. Data from three independent experiments are compared with theoretical model simulations.