Myo1b is associated with and propagates with actin waves. (A) Merged fluorescence images acquired with SIM of an actin wave costained with anti-Myo1b antibodies, Alexa Fluor 488 phalloidin, and anti–β3-tubulin antibody and images of the 2.5× enlargement of the region marked by a white box are shown. (B) The propagation of F-actin structures and mCherry-Myo1b has been analyzed in cortical neurons expressing LifeAct-EGFP and mCherry-Myo1b at DIV1 by time-lapse spinning confocal microscopy. The first frame of Video 1 (one frame per minute) for LifeAct-EGFP and mCherry-Myo1b and the merged kymographs of 36 frames for the region marked on the first frame by a white lane for the two recombinant proteins are shown. The increase of fluorescence intensity for LifeAct-EGFP correlated with the increase of fluorescence intensity for mCherry-Myo1b. (C) The propagation of PIP3 and mCherry-Myo1b has been analyzed in cortical neurons expressing AKT-PH-EGFP and mCherry-Myo1b at DIV1 by time-lapse spinning confocal microscopy. The first frame of Video 2 and kymographs of 36 frames for the region marked on the first frame by a white lane for the two recombinant proteins are shown. The increase of fluorescence intensity for AKT-PH-EGFP correlated with the increase of fluorescence intensity for mCherry-Myo1b. Bars: (A) 10 µm; (B and C) 20 µm. T, time