PREM of VE-cadherin distribution after CK666 treatment. (A and F) Overviews of the cell–cell interfaces with pseudocolored individual cells. Gaps between cells remain gray. Outlined regions are enlarged in B and G, respectively. (B) VE-cadherin immunogold (18 nm, yellow) labels both sides of the intercellular gap. The region outlined in white was analyzed at high magnification to locate gold particles. The region outlined in cyan is enlarged in C. (C and G) VE-cadherin is associated with the gap edges (C) and intercellular bridges (G). (D and E) VE-cadherin immunogold at gap edges associates with nonfilamentous material (shaded red in D) residing on the actin bundle. The boxed region in D is enlarged in E. Bars: (A and F) 5 µm; (B) 2 µm; (C–E and G) 200 nm.