Orientation of actin branches at linear AJs. (A) Overview of a linear AJ between two cells. The boxed region is enlarged in B. (B) Lamellipodia from the attacking cell (shaded in yellow) collides with the tangential bundle of the defending cell (blue). Boxed regions are enlarged in C and D, respectively. (C and D) Double-immunogold labeling of NMIIA (12 nm, cyan) and VE-cadherin (18 nm, yellow). Actin branches at linear AJs contain two oppositely oriented sets. VE-cadherin immunogold is mostly located between these sets. Representative branches presumably originating from the blue and yellow cells are shaded in red and green, respectively; branches with neutral orientation are shaded in blue. Bars: (A) 5 µm; (B) 1 µm; (C and D) 100 nm.