Localization of VE-cadherin and α-catenin at linear AJs. (A) Linear AJ labeled with VE-cadherin (18 nm, yellow) and NMIIA (12 nm, cyan) immunogold. The region outlined in white was analyzed at high magnification to locate gold particles. The region outlined in green is enlarged in B. (B) VE-cadherin immunogold (yellow) colocalizes predominantly with the branched strip even as the strip shifts from one bundle to another (arrow). Ends of oblique actin bundles near the branched strip are also occasionally labeled with VE-cadherin immunogold (arrows in A). NMIIA immunogold (cyan) labels tangential and oblique actin bundles. (C) VE-cadherin (18 nm, yellow) and α-catenin (12 nm, magenta) immunogold colocalize with each other on the branched actin network. Bars: (A) 2 µm; (B and C) 200 nm.