PREM of linear AJs. (A) Overview of a cell (shaded in brown) that forms linear AJs with three neighboring cells (green, blue, and cyan) and also contacts the fourth cell (red). The boxed region is enlarged in B. (B) Linear AJ contains a narrow strip of branched network (orange) flanked by two actin-NMII bundles (cyan). Boxed regions are enlarged in C and D, respectively. (C and D) NMIIA immunogold (18 nm, cyan) and some actin branches (D, orange) are pseudocolored. Bars: (A) 10 µm; (B) 1 µm; (C and D) 200 nm.