Fluorescence microscopy of actin cytoskeleton components at AJs in HUVECs. (A–C) Costaining with phalloidin (red) and antibodies to VE-cadherin (yellow) and VASP (cyan). (D–F) Costaining with phalloidin (red) and antibodies to NMIIA (yellow) and VASP (cyan). Boxed regions in A and D outline linear (b and e) and punctate (c and f) AJs and are enlarged in B, C, E, and F, respectively. Arrowheads in B and E mark linear AJs. Bars: (A and D) 10 µm; (B, C, E, and F) 5 µm.