Plk4 levels influence the rate and period of centriole growth. (A) Graphs compare the mean Sas-6-GFP incorporation profile during nuclear cycle 12 in WT embryos (orange) and Plk4^1/2 embryos (brown). (B) Charts quantify parameters of centriole growth derived from the Sas-6-GFP incorporation profiles in A. (C–F) Graphs and charts show the same analysis for Plk4^2x (dark brown) and Plk4^RKA embryos (gray). Data are represented as mean ± SD (n ≥ 15 embryos for each group). Statistical significance was assessed using either an unpaired t test with Welch’s correction (for Gaussian-distributed data) or an unpaired Mann–Whitney test (*, P < 0.05; ***, P < 0.001; ****, P < 0.0001; ns, not significant). A.U., arbitrary units. n ≥ 15 embryos for each group; n = 92, 84, 98, and 39 centrioles (mean) from each WT, Plk4^1/2, Plk4^2x, and Plk4^RKA embryo, respectively.