Figure 6. G3BP1 and IMP1 have a short... | Rockefeller University Press

Figure 6.

$Figure 6. G3BP1 and IMP1 have a short lifetime in the bound fraction and display anomalous subdiffusion in the mobile fraction. (A) Lifetime determination of different combinations of HaloTag- and SNAP-tagged G3BP1 and IMP1 in the bound fraction of SGs. Trajectories of the respective Halo-tagged constructs (shown in bold) are evaluated. (B) Example of the trajectories of the mobile fraction of HaloTag-G3BP1 within a single granule (left) and plot of the MSD against the elapsed time showing anomalous diffusion. Mean diffusion constants (Γ) and α values of the respective HaloTag construct in the mobile fraction as determined for the different combinations. Bar, 500 nm. Calculations were performed on the mobile trajectories of 8–11 SGs per experimental condition as indicated by n. SGs were obtained from 8–11 cells from two to eight independent experiments. For all experiments, stress had been induced by a 20-min treatment with 0.5 mM sodium arsenite before imaging.$

G3BP1 and IMP1 have a short lifetime in the bound fraction and display anomalous subdiffusion in the mobile fraction. (A) Lifetime determination of different combinations of HaloTag- and SNAP-tagged G3BP1 and IMP1 in the bound fraction of SGs. Trajectories of the respective Halo-tagged constructs (shown in bold) are evaluated. (B) Example of the trajectories of the mobile fraction of HaloTag-G3BP1 within a single granule (left) and plot of the MSD against the elapsed time showing anomalous diffusion. Mean diffusion constants (Γ) and α values of the respective HaloTag construct in the mobile fraction as determined for the different combinations. Bar, 500 nm. Calculations were performed on the mobile trajectories of 8–11 SGs per experimental condition as indicated by n. SGs were obtained from 8–11 cells from two to eight independent experiments. For all experiments, stress had been induced by a 20-min treatment with 0.5 mM sodium arsenite before imaging.