CCR2 inhibition blocks the increased leukocyte infiltration in VASP^−/− mice after hindlimb ischemia. (A) Time protocol showing the daily injection of the CCR2 inhibitor RS504393 (+RS; 4 mg/kg per day) or vehicle (+VH) from 2 d before until 2 d after femoral artery excision. Leukocyte infiltration into ischemic and control calf muscles was analyzed by FACS 3 d after hindlimb ischemia induction. (B–D) Quantification of leukocyte numbers isolated from calf muscles per gram of tissue 3 d after ischemia induction: neutrophils (B), monocytes (C), and M1-related macrophages (D). n = 6 animals per group; error bars, SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns = nonsignificant (two-way ANOVA/Bonferroni).